History 1920s Essay Example for Free

History 1920s Essay America went through many social changes with the American Revolution and industrial revolution. During the 1920’s, the face of America began to change more into an urban society. Many differences between the rural and urban sides of America emerged. Historians consider the tensions of the 1920s as a backlash against the rising urban America, which turns out true. Rural people believed that the city lacked morals. The urban city dwellers lashed back saying that rural residents did not understand the technology of modern times. In the 1920s, for the first time in American history, more people lived in cities rather than the countryside. People living in the more rural parts of America saw the urban lifestyle as a threat to traditionally shared value. The cities, a haven for political corruption and distraction, had much more alcohol and prostitution problems than their countryside counterparts did. With the rise of Hollywood and films, people found an escape from their routine life and went to see movies that portrayed what it really meant to live the American dream. However, the rural side of the country stuck to the old ways of agriculture life as the struggle between old and new still went on. With the rise in media also came an increase of cultural battles. The media brought about a revolution of morals since many urban Americas saw the changes in media and lifestyle as liberation from the old countryside Victorian past. Granted, the rural Americans did not see it this way. They felt that American had begun to change in sinful ways and that the ethicality of America had begun to decay. Many people in the countryside also resented the amount of immigrants coming into America. As a result, the Klu Klux Klan revived and revolted against the immigrants, Blacks, Catholics, Jews, and any modern or progressive movement. Located mostly in the rural parts of America, the Klu Klux Klan greatly showed their discontent with the modern American lifestyle. The South showed their animosity towards the foreigners and Blacks through discrimination and racism. However, none of this stopped the urban rise of America that the country would soon have to accept.

The Beginning of Autumn :: essays research papers

The beginning of autumn It had been raining all day, and Irma had started to feel the symptoms of the flu. She always suffered during the season of allergies, and autumn always brought dust with it. At the end of the day, Enrique, her husband, came home and asked her to accompany him to Monterrey the next day. He was in the middle of a big deal of his professional career, and someone wanted him over there taking charge of his business, it was imperative he attend that appointment! It was the only opportunity he had or they would hire someone else for the new position. In her rushed packing she included some newborn’s clothes into their baggage by mistake. She had planned to stop by the hospital to pay the balance for her new daughter’s delivery scheduled the following month, but she wouldn't have time. They woke up early in the next morning and she felt worse with her flu and allergies, so she decided to take some medicine, â€Å"once we got there, I will be ok, and then we'll come back, and I will be able to rest† she thought. They arrived at the bus station and on the way Enrique bought a newspaper. The top heading read move to â€Å"NY Mets go into 1st place after trailing for 12 games†(citation. He got excited because his father had always followed the Mets very closely (Escobar). They arrived around 5:00 in the morning, and stopped for something to eat. Enrique’s appointment was schedule for 9:00 a.m., so they had enough time to get breakfast. She got out of the car, and she felt a little dizzy, but she didn't give it any importance. They entered the restaurant, and she asked for her favorite meal, â€Å"Machacado con huevo†. As she was about to take the first bite, she felt water between her legs. â€Å"Oh my God!!! Its time†, she screamed. Enrique paid the check without eating anything and asked for the closest hospital. As soon as Irma got into the car, she fainted. Enrique was so nervous that he overlooked the first hospital in sight and kept driving. Finally they reached a hospital and the helped him to get Irma inside. They didn’t even register but went directly into the delivery room. It took more that three hours to pull her child out; Irma was unconscious. Finally, the clock announced nine o’clock and he then realized that he has missed the appointment, yet the sound of the new baby’s screaming got him from his thoughts, it was their baby! It was a chubby baby girl that weighted 9 pounds!

Chromatography for Protein Purification

Department of Chemical & Biomolecular Engineering THE NATIONAL UNIVERSITY of SINGAPORE Chemical Engineering Process Laboratory II Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification.B. Introduction I. Fast Protein Liquid Chromatography (FPLC) High Performance Liquid Chromatography (HPLC) is the workhorse for any biopharmaceutical protein downstream processing train, featuring at least twice within the train. You must recall experiencing the HPLC in one of the experiments in your CN2108 module. Read up on the essential parts of the HPLC system. In this experiment, you will use a modification of the HPLC, the FPLC (Fast Protein Liquid Chromatography System) to separate and purify a mixture of two proteins.The FPLC has been developed to specifically take advantage of the resolution capability of the HPLC for protein purification and collection. II. Concepts in LC When a mixture of proteins is injected into an LC column, the proteins interact with the stationary phase based on their respective chemistries and move through the column at different speed. Based on this differential migration, the proteins elute from the end of the column at different times and therefore become separated. This process is usually facilitated by following the proteins with a mobile phase.Although the protein mixture will have entered as a narrow, concentrated peak, it will exit dispersed and diluted by the mobile phase. This is called bandspreading. Bandspreading (which is an inverse indication of the column efficiency) must be minimized especially for large-scale protein purification. When bandspreading is severe, the proteins may not be sufficiently resolved within a reasonable time-frame. The degree of separation of one component from another is referred to as the resolution (RS), determined based on equation 1 (refer to Fig. 1): RS = VB ? V A 0. (W A + WB ) †¦Eqn. (1) Injection wA VA VB wB Figure 1. Typical protein chromatogram Note that resolution can also be defined based on retention times, instead of volumes. There are various ways to improve resolution, the most straightforward of which is to vary the mode of elution – isocratic versus gradient. Both of these modes are based on the strength of the mobile phase, which directly affects the interactions between the proteins and the stationary phase. In protein chromatography, it is desirable to have high yield as well as high purity of the collected fraction.Yield is the amount of a protein collected as a fraction of the total amount of the same protein fed, while purity is a measure of how much of that protein is in the fraction collected. C. Experimental I. Protein Quantification You will design an experiment to obtain the calibration curves for the two proteins provided using FPLC. You are provided with the following for this experiment: 1. An FPLC system which has been properly set up and equilibrated. You only need to inject 100  µL of each of your samples, and your data will be recorded and analysed by the computer.Note the profile of the mobile phase programmed. 2. A protein mixture containing two proteins (S1 and S2) at concentrations of 1. 0 mg/mL each. II. Protein Purification and Collection You are to perform a chromatographic purification of 1mL of the protein mixture provided. You can expect the chromatogram shown in Fig. 2. Myoglobin Lysozyme Figure 2. Chromatogram of two proteins from FPLC Based on Figure 2, you are to conduct the following: 1. Collect one fraction of the highest yield that is 100% pure S1, and the balance in another fraction. 2.Collect one fraction containing a s much of S1 fed as possible. D. Discussion 1. 2. Briefly describe the experiment that you designed in CI. Explain your choice of the collection times for each of your collection in experiment CII. Determine the yield and purity of each of your collected fractions. Perform a material closure for each of CII (1) and CII (2). Based on your results in (2) above, rationalize the importance of resolution in chromatographic separations. Given the tension between yield and purity, which, in your opinion, is more crucial, yield or purity?How do you propose to improve the resolution of S1 and S2 in this chromatographic purification? 3. 4. Useful Notes 1. Reagents: a. Mixture of two proteins b. Mobile phase – 2M ammonium sulfate in 100mM Phosphate buffer pH 7. 0 c. Elution – 100mM Phosphate buffer pH 7. 0 FPLC to be set up with the appropriate parameters: a. detection wavelength at 280nm b. sample loop – 100  µL c. HIC column for protein separation. d. mobile phase â₠¬â€œ 2 M ammonium sulfate e. mobile phase flow rate: 1 mL/min f. gradient elution – linear gradient 100% to 0% over 10 column volume. 2.

Theological Differences Between Martin Luther, Ulrich...

THEOLOGICAL DIFFERENCES BETWEEN MARTIN LUTHER, ULRICH ZWINGLI AND JOHN CALVIN INTRODUCTION The purpose of this essay is to discuss the significant theological differences that occurred between Martin Luther, Ulrich Zwingli and John Calvin. To gain a greater understanding of their theological differences it must first be discovered who these prominent men were. Once this is achieved why they longed for reformation will be discovered. In doing so many similarities in what they were trying to bring in the form of reform will be shown. After this a detailed understanding of their major 33differences will be shown. It will be discovered that these differences occur around the understanding of the Eucharist. This will lead ultimately to the†¦show more content†¦As a result a rift began between Luther and Zwingli. Prince Phillip Hesse attempted to come to a solution of disagreement between the two parties. As a result two wars of Capel would begin. The first had no bloodshed. The second led to Zwingli being defeated and dying in 1531. Zwingli had many similar ideas as t o Luther but his ideas especially concerning the Eucharist were much more radical which ultimately led to his death. Presbyterian theology could not have developed without the efforts of Zwingli. He was a true pioneer and reformer of his day. JOHN CALVIN (1509-1564) John Calvin was known as a theologian and French reformer. He studied arts at the college de Montaigu. 1528 he studied civil law at Orleans and later at Borges. Here he discovered the ideas of humanism. 1531 Calvin returned to Paris to study letters and publishing his commentary in 1532. In 1533 due to his ideas regarding reform he fled Parris. He would eventually end up in Basle. In 1536 he was convinced by G. Farel to stay in Geneva and help in organization of the reformation. His ideas would soon have him moved on to Strasbourg where he was Pastor for another three years. In 1541 he returned under invitation to Geneva where he would stay for a further fourteen years. His wife died in 1549 leaving him to raise two children. By 1555 opposition to Calvin and his views would cease. In 1559 he wasShow MoreRelatedMartin Luther And The Protestant Reformation1461 Words   |  6 Pagespersecutions. In northern and central Europe, reformers like Martin Luther, Thoma s Muntzer, Ulrich Zwingli, John Calvin and Henry VIII challenged papal authority and questioned the Catholic Church’s practices, such as â€Å"indulgence† and â€Å"enough good works to merit salvation† (Sayre 263-266). In Who was Martin Luther Whose 95 Theses Helped Spark the Reformation? (October 31st 2017), Anderson Mike Ellis looks specifically at Martin Luther and his belief and why he wrote the Ninety-Five theses, whichRead MoreRenaissance And Reformation, 1350-16001879 Words   |  8 Pagesbut correctly marked the period of the European history. The word in French meant ‘rebirth’ of the European civilization. The Reformation was the break from the Catholic Church and the birth of Lutheranism or Protestant that was championed by Martin Luther King. The essay will focus on the origin of Renaissance and Reformation explaining the intellectual and artistic renaissance. It will also explain the Protestant Reformation and the spread of Protestant and the Catholic response. The Renaissance